This paper is about the use of perceptual principles for melody estimation. The melody stream is understood as generated by the most dominant source. Since the source with the strongest energy may not be perceptually the most dominant one, it is proposed to study the perceptual properties for melody estimation: loudness, masking effect and timbre similarity. The related criteria are integrated into a melody estimation system and their respective contributions are evaluated. The effectiveness of these perceptual criteria is confirmed by the evaluation results using more than one hundred excerpts of music recordings.