Musical interpretation is an intricate process due to the interaction of the musician’s gesture and the physical possibilities of the instrument. From a perceptual point of view, these elements induce variations in rhythm, acoustical energy and timbre. This study aims at showing the importance of timbre variations as an important attribute of musical interpretation. For this purpose, a general protocol aiming at emphasizing specific timbre patterns from the analysis of recorded musical sequences is proposed. An example of the results obtained by analyzing clarinet sequences is presented, showing stable timbre variations and their correlations with both rhythm and energy deviations.